SYLLABUS:
Biochemistry
-NB560 Advanced Biochemistry topics I (Credits: 3)
Syllabus: Reporting and discussion of results from studies and recent literature regarding specific research areas of the program.
- NB565 Advanced Biochemistry topics II (Credits: 3)
Syllabus: Reporting and discussion of results from studies and recent literature regarding specific research areas of the program.
Physiology
- NF021 Introduction to Molecular Biology (Credits: 3)
Syllabus: General organization of the genome of eukaryotes. Basic principles of conventional techniques of molecular biology for: identification of target genes, study of gene expression, and study of gene transcription. Presentation and discussion of different examples of the interaction of environmental factors with gene expression, at transcriptional and post-transcriptional level: genes responsive to nutrients, drugs, hormones, temperature, etc. Stryer -Biochemistry - Freeman; Alberts, Bray, Lewis, Raff, Roberts, Watson - Molecular Biology of the Cell - Garland; Garrett, Grisham - Molecular Aspects of Cell Biology - Saunders College; Watson, Witkowski, Gilmar, Zoller - Recombinant DNA - Scientific American Books; Lewin - Genes VI - Oxford; Wilson, Foster, Kronenberg, Larsen. Williams Textbook of Endocrinology, WB Saunders SB Farah - DNA: secrets and mysteries - Savier; specific texts and articles for each class.
- NB515 Molecular Bases of Gene Expression (Credits: 3)
Syllabus: Genome - Replication and repair - Oncogenes - Transcription and post-transcription processing - Translation and post-translational processing - Addressing protein - Antibiotic and Chemotherapy drugs - Regulation of gene expression - Genetic recombination - PCR - Cloning – Apoptosis.
- NF122 Special topics in Physiology (Credits: 3)
Syllabus: Presentation and discussion of experimental and literature results related to research areas of the program.
Common Core
-NB580 Seminars on Functional and Molecular Biology I (Credits: 3)
Syllabus: Presentation of research topics of interest by guest postgraduate students or professors.
-NB581 Seminars on Functional and Molecular Biology II (Credits: 3)
Syllabus: Presentation of research topics of interest by guest postgraduate students or professors.
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Optional disciplines
- NB171 Biochemical Bases of Plant Defense Mechanisms (Credits: 3)
Syllabus: Basic concepts of plant resistance; types of resistances; structural defenses; secondary metabolites; primary metabolites; biochemical aspects of induced defenses; plant improvement for plant resistance to insects and pathogens.
- NB192 Seminars of the National Laboratory of Biosciences (Credits: 3)
Syllabus: Seminars on scientific advances in studies on molecular and structural biology.
- NB211 Enzymology (Credits 7)
Syllabus: Purification of enzymes - Enzyme kinetics - Inhibitors - Reactions with more than one substrate. Mechanism of enzymatic reactions. Methods to determine enzyme activities - enzyme-substrate interactions - enzyme systems.
Bibliography: 1) Dixon, M. and Webb, E.C. (1979). Enzymes, 3rd Ed. Academic Press, N.Y., 2) Scopes, R.K. (1982). Protein Purification. Principles and Practice. Springer-Verlag, N.Y., 3) Deutcher, M.P. (1990). Guide to Protein Purification. Methods in Enzymology, vol 182. Academic Press, N.Y., Collection - Methods in Enzymology.
- NB282 Higher Education Methodology – Field: Biology (Credits: 6)
Syllabus: Introduction to basic pedagogical ideas of Piaget, Rogers, Skinner, and Neil. Discussion of the curriculum (and its main components: content, methodology and evaluation) of Biochemistry disciplines and other disciplines of the biological area, for undergraduate courses. Application of concepts developed in theoretical and practical classes.
- NB283 Tumor Metabolism (Credits: 2)
Syllabus: Metabolic reprogramming and cancer. - Relationship between catabolic and anabolic pathways in cancer. - Metabolic flexibility and cancer progression. - Metabolic symbiosis: relationship between the metabolism of tumor cells and their microenvironment. - Use of tumor metabolism for the development of therapeutic and diagnostic strategies. - Experimental strategies for the study of tumor metabolism.
Bibliography: The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. DeBerardinis RJ, Lum JJ, Hatzivassiliou G, Thompson CB. Cell Metab. 2008 Jan; 7(1):11-20. Metabolic reprogramming: a cancer hallmark even warburg did not anticipate. Ward PS, Thompson CB. Cancer Cell. 2012 Mar 20; 21(3):297-308. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Vander Heiden MG, Cantley LC, Thompson CB. Science. 2009 May 22; 324(5930):1029-33. Aerobic glycolysis: meeting the metabolic requirements of cell proliferation. Lunt SY, Vander Heiden MG. Annu Rev Cell Dev Biol. 2011;27:441-64. Targeting cancer metabolism: a therapeutic window opens. Vander Heiden MG. Nat Rev Drug Discov. 2011 Aug 31;10(9):671-84. Targeting metabolic transformation for cancer therapy. Tennant DA, Durán RV, Gottlieb E. Nat Rev Cancer. 2010 Apr;10(4):267-77. Rocking cell metabolism: revised functions of the key glycolytic regulator PKM2 in cancer. Chaneton B, Gottlieb E. Trends Biochem Sci. 2012 Aug; 37(8):30. 9-16.
- NB325 Experimental Biochemistry I (Credits: 4)
Syllabus: Use of specific techniques for levels of the protein structure organization. Conformational models, dynamic processes in biological membranes, mechanisms of biological catalysis, structural stability of nucleic acids. Process of energy transduction in the cell.
- NB326 Experimental Biochemistry II (Credits: 4)
Syllabus: Use of specific techniques for levels of the protein structure organization. Conformational models, dynamic processes in biological membranes, mechanisms of biological catalysis, structural stability of nucleic acids. Process of energy transduction in the cell.
- NB510 Biochemical and physiological processes of cell metabolism (Credits: 6)
Syllabus: Correlation of cell biology with biochemistry. - Biological membrane transport mechanisms. - Importance of regulatory enzymes in metabolism. - Absorption and utilization of nutrients by the cell. - Cell signaling. - Integrated function of tissues under physiological and pathological conditions.
- NB520 Special Biochemistry topics (Credits: 3)
Syllabus: Presentation and discussion of experimental and literature results related to research areas of the program. Complement of theoretical training in specific area.
- NB523 Biomembranes (Credits: 6)
Syllabus: Introduction to biomembranes: composition, movements of components, functions, membrane models. Transport and other phenomena occurring in the membranes: phase transition, fusion, endocytosis/exocytosis, synthesis and metabolism of membrane components, interaction with amphiphiles and its consequences. Methods applied to the study of membranes.
- NB530 Teaching activity (Credits: 6)
Syllabus: With the professor’s guidance, the student helps plan a particular undergraduate course, teaches classes, prepares didactic material, performs support activities, and plans practical classes.
- NB531 Software development for Biochemistry and Physiology classes (Credits: 2)
Syllabus: The objective of the discipline is to promote the development of the critical sense of future teachers regarding the use of software in higher education, particularly in the areas of Biochemistry and Physiology. Planned activities: 1) evaluation of educational software; 2) software development planning; 3) use of digital content editing tools.
- NB550 Class planning I (Credits: 6)
Syllabus: Complete planning of an elective discipline for undergraduate program based on a topic of interest that has not been explored in regular disciplines.
- NB555 Class planning II (Credits: 6)
Syllabus: Application of planned discipline to discipline NB550.
- NB556 Enzymology and Biotechnology topics (Credits: 4)
Syllabus: Presentation and discussion of theoretical methodological questions and of recent literature to the areas of Enzymology and Biotechnology applied to bioproducts.
- NB557 Mass spectrometry-based proteomics (Credits: 5)
Syllabus: This discipline covers topics such as principles of proteomics and mass spectrometry, preparation of samples for large-scale analysis, protein and peptide fractionation and enrichment methods, protein interaction analysis, quantitative proteomic methods with and without peptide labeling, post-translational modifications, instrumentation, peptide fragmentation methods, data processing using analysis platforms, and functional annotation of proteomes.
Bibliography: Protein sequencing and identification using tandem mass spectrometry, Michael Kinter and Nicholas Sherman, and articles published in this field and suggested to students.
- NF012 Neuronal interaction (Credits: 3)
Syllabus: Interaction processes between neurons: mechanisms of synapse. Chemical processing in synapses. Neural plasticity. Control of neurovegetative functions. The neurovegetative system and its divisions (sympathetic, parasympathetic and enteric systems). Neurovegetative integration processes: hypothalamus and limbic system: modulation of motivational processes and behavior.
Bibliography: PURVES, D. et al., Neurociências. Artmed, 4ª ed., 2010. LENT, R. Cem Bilhões de Neurônios? Conceitos Fundamentais de Neurociência. Atheneu, 2ª ed., 2010. KANDEL, E. R.; SCHWARTZ, J. H.; JESSEL, T. M.; SIEGELBAUM, S. A.; HUDSPETH, A. J. Princípios de Neurociências. Artmed, 5ª ed., 2014.
- NF015 Cardiovascular function (Credits: 3)
Syllabus: Cardiac Dynamics. Electrocardiography. Hemodynamics. Circulations: systemic, pulmonary and in special regions. Cardiovascular regulation at rest and in pathophysiological conditions.
- NF016 Physiology and Biophysics topics I (Credits: 3)
Syllabus: Analysis and discussion of theoretical methodological questions and experimental data related to the areas of Physiology and Biophysics.
- NF017 Fundamentals and Updates of Pharmacokinetics (Credits: 3)
Syllabus: Fundamentals and discussion of data analysis related to the models of drug absorption, distribution, metabolism, and excretion in the body. Pharmacokinetic parameters and bioavailability and bioequivalence.
Bibliography: Makoid MC, Banakar UV and Vecutech, “Basic Pharmacokinetics”, www:Creighton.edu/pkinbk/psc443; Scientist, ver. 4.0, Micromath, Salt Lake City, UT (disponibilizado); Pkinetics, ver. 3.0, Micromath, Salt Lake City, UT (disponibilizado); Ritschel, WA, et.al., “Handbook of Clinical Pharmacokinetics”, ASHP, Washington, DC (2002).
- NF103 Reproduction (Credits: 3)
Syllabus: Gonads. Testicles and ovaries. Puberty. Fertilization and implantation. Gestation. Placenta. Childbirth. Breastfeeding. Contractility of the normal and pregnant uterus. Fetus and newborn. Contraceptives.
- NF110 Hormone signal transduction (Credits: 3)
Syllabus: Hormones. Receptors. Feedback. Hypothalamic-pituitary axis. Adenohypophysis. Neurohypophysis and Pars Intermedia. Pineal. Thymus. Parathyroid. Pancreas. Adrenals: Cortex and Medullary. Thyroid.
- NF114 Functional Analysis of Behavior (Credits: 4)
Syllabus: Behavior studied through direct observation and experimental method. Operant and corresponding behavior. Cognitive processes and animal behavior. Physiological processes and behavior.
- NF116 Physiology and Biophysics topics II (Credits: 3)
Syllabus: Analysis and discussion of theoretical methodological questions and experimental data related to the areas of Physiology and Biophysics.
- NF132 Fundamentals of Applied Biostatistics (Credits: 3)
Syllabus: After recalling concepts such as population; sample; distribution; mean; median; fashion; standard deviation and standard error; interquartile range;, continuous, discontinuous, qualitative and quantitative variables, parametric and nonparametric statistical tests will be applied using a computerized statistical package.
- NF133 Scientific Methodology (Credits: 3)
Syllabus: Discussion of general subjects from sciences: philosophy, ethics, history, dogma and paradigm in science.
- NF135 Carbohydrate metabolism and glycemic control (Credits: 03)
Syllabus: Carbohydrate metabolism, energy demand and contribution of different tissues, integration of metabolism and hormone action, states of resistance to hormone action, metabolic diseases.
Bibliography: -WILSON JD & FOSTER DW. Williams (2008) Textbook of Endocrinology. th. ed., Philadelphia, Saunders. -SHILS ME, OLSON JA, SHIRE M & ROSS AC. (1999) Modern Nutrition in Health and Disease. 9 th. Ed., Baltimore, Williams & Wilkins. -ZUBAY, G. (1993) Biochemistry. 3 th. Ed, Dubuque, Wm.C. Brown Publishers. -LEHNINGER AL, NELSON DL, COX MM. Principles of Biochemistry, 2nd ed, New York, Worth Publishers. -LeROITH D, TAYLOR SI, & OLEFSKY JM. Diabetes Mellitus, 1st ed., Philadelphia, Lippincott – Raven. -FLATT P & LENZEN S. Frontiers of insulin secretion. 1st ed., Great Britain, Smith-Gordon NISIMURA. -STRAUSS RH. Sport Medicine. 2nd ed., Philadelphia, W. B. Saunders Company. –LITERATURE ARTICLES SUGGESTED BY THE PROFESSORS IN CLASS.
- NF138 Bioethics (Credits: 4)
Syllabus: Origin of the term Bioethics; Concepts of ethics, moral, legality and justice; Scientific-technological development and ethical dilemmas; Bioethics and health sciences; Teaching Bioethics; Bioethics and scientific research; International and national regulations in research with human beings; Ethical principles in animal experimentation; Research ethics committees; Evaluation of research protocols by ethics committees.
- NF139 Progress in lipid metabolism (Credits: 5)
Syllabus: Lipid metabolism of different tissues of the body, with emphasis on adipose tissues and intravascular compartment. It addresses advances in specific knowledge about the control and regulation of the following processes: adipogenesis, lipogenesis, lipolysis, transport of lipids, and the effects of disorders on these processes, such as obesity and atherosclerosis.
Bibliography: Williams Textbook of Endocrinology, and recent reviews of specific literature.
Note: Objectives: Make the student develop advanced bibliographic research on a specific topic in the field of lipid metabolism, so that the student can develop a critical view and present the “state of the art” on the subject. Strategies: Theoretical classes and discussions conducted by the professor. Assessment criteria: the students should present a topic in the form of a seminar and a monograph. Their ability of topic presentation and discussion will be self-evaluated, and evaluated by classmates and the discipline professor he teacher of the discipline.
- NF140 Development of scientific articles (Credits: 4)
Syllabus: Identify proper journals to publish the results obtained, prepare the article following the journal guidelines, including the letter of manuscript submission.
Bibliography: Código de boas práticas científicas (Good scientific practices), FAPESP, v. 05/09/2011
Note: The journal selection and the manuscript development should be conducted in agreement with the student’s advisor.
- NF141 Teaching and evaluating Physiology (Credits: 4)
Syllabus: Prepare and identify the main concepts to be presented in a 50-minute class at the undergraduate level. Develop a critical view of the content to be taught, creating detailed insights of the classes.
Bibliography: Fisiologia Básica, by Rui Curi & Joaquim Procópio, Guanabara Koogan, 2009. GUYTON, A.C. & HALL, J.E. Tratado de fisiologia médica. 12ª ed. Elsevier, Rio de Janeiro, 2011.
- NF142 Cell signaling in vascular tone control (Credits: 3)
Syllabus: This topic will be address, through conferences and presentation of scientific studies, advances in the knowledge of cell signaling mechanisms related to factors involved in vascular tone control: endothelial factors; perivascular tissue; calcium; protein kinases and phosphatases; reactive oxygen species; renin-angiotensin-aldosterone system; adrenergic receptors; alteration to these mechanisms in cardiovascular diseases.
Bibliography: Recent articles of the literature in this field.
- NG252 Genomics and Biotechnology (Credits: 8)
Syllabus: This discipline aims to (1) present the technical and scientific principles of genome projects, including a strategic view of how the analyzed organisms are selected; (2) update students in relation to all genome projects conducted so far; (3) present the principles to obtain resources for projects; (4) present the principle of creation and operation of biotechnology companies, connecting it with venture capital and stock exchange. Methodology: Theoretical classes covering technical principles, followed by seminars on genome projects conducted so far, ending with theoretical classes and presenting the funding principles for venture capital, and initial public offering in stock exchanges.